Tommaso Selmi

Ricercatore

E-Mail

tommaso.selmi@cnr.it

PHONE

none

LOCATION

Segrate

ROOM (floor/number)

7/24

My laboratory integrates functional genomics, ribosome profiling, and sequencing approaches to study RNA biology, with a particular focus on RNA modifications and their impact on translation. Through this approach, I aim to define RNA-dependent mechanisms that sustain proliferative capacity in stem cells and cancer. I use CRISPR base-editing to dissect RNA modification–associated effectors that support tumor proliferation, drug resistance, and represent potential therapeutic vulnerabilities.

Research Activities

Study of the role of tRNA modifications and tRNA-derived fragments in regulating translation to control stem cell self-renewal and differentiation

Investigation of how specific RNA modifications and ribosome translation dynamics together influence stem and cancer cell proliferation and fate decisions

Determination of how Single Nucleotide Variants (SNVs) in RNA-modification effectors influence cancer cell proliferation and drug resistance

Development of high-throughput CRISPR screening platforms, integrating machine learning-guided library design with large-scale genetic perturbation experiments in different biological contexts

Characterization of the functional landscape of single nucleotide variants (SNV) in disease models by using large-scale CRISPR screens and quantifying variant effects via phenotypic and transcriptional readouts

Study of molecular and cellular mechanisms in tumorigenesis with a focus on dissecting cellular heterogeneity and interactions within the tumor microenvironment

Projects